We present a filter-less time-resolved fluorescence (TRF) measurement system using an optoelectronic CMOS sensor for high-sensitivity readout of LFA test strips. The TRF measurement sequence and timing regime were implemented in an FPGA. The CMOS sensor ASIC-based TRF reader was validated using Eu chelate particle-labeled human CRP as a model analyte in comparison to a commercial colorimetric LFA reading system. The filter-less TRF reader showed a DDR of 4 orders of magnitude with a LoD of 0.2 ng/ml for quantification of CRP, which was 3.5 times better than the LoD of the commercial LFA reader.