This paper presents a highly digital bio-impedance measurement system-on-chip, which can convert the magnitude and phase of impedance to 10-bits digital codes integrated with a filter-based wide-range programmable sinusoidal wave synthesizer (SWS). The proposed SWS utilizes switched-capacitor circuits such that the corner frequency can be adjusted by changing its switching frequency. The proposed EIS system was fabricated using 180nm process with an active area of only 0.35 mm2. The SWS generates output frequency in the range of 579 µHz–48.9 kHz with measured total harmonic distortion of 0.152% at 100 Hz and 0.116% at 10 kHz. The DNL of the proposed magnitude converter is within −0.24/+0.2 LSB, and the INL is around −0.75/+1 LSB. To validate the function of the proposed EIS measurement system, it is utilized to detect the tumor necrosis factor α in biological samples.