There is a hypothesis that ISFET-based DNA detection could be enhanced by positioning DNA close to the ISFET surface using electrodes that manipulate DNA through dielectrophoresis (DEP). This paper provides a proof-of-concept for an ISFET-DEP system for DNA detection, combining information gathered from the literature and FEM electric field simulations to test the validity of the hypothesis and highlight key challenges in implementation. An electrode system was designed with sets of line and rectangular castellated interdigitated electrodes that is capable of trapping DNA at low voltage. Finally, a 180nm CMOS prototype was developed for preliminary testing.