The existing CMOS platforms monitor the bacterial growth using single mode sensor and the bacteria samples are prepared separately which cause unreliable sensing results and potential contamination. In this paper, we present a CMOS multi-sensor array features high sensitive hydrogen ion, optical sensing and temperature modulation capability for accurate on-chip bacterial growth monitoring. A novel high sensitive hydrogen ion sensor and image sensor are integrated with an in-pixel capacitive transimpedance instrumentation amplifier (CTIA) to achieve both pH and optical sensing functionalities. The temperature modulation for on-chip bacteria culturing is realized through temperature sensor, heater and control circuit. A column SAR-Single Slope dual-mode ADC is employed, considering a quantization speed, chip area compromise and its compatibility with correlative double sampling. A state-of-the-art pH sensitivity of 217mV/pH is achieved in simulation. The high sensitive and multi-sensing features also make this sensing platform a promising candidate for antibiotic study, DNA sequencing, etc.