Near-infrared fluorescence image-guided surgery combines near-infrared fluorescent markers and near-infrared sensitive cameras to map relevant structures during surgical procedures. As targeted near-infrared fluorophores are evaluated for clinical applications, near-infrared imagers are not being developed with the architectural improvements and optoelectronic characteristics that will facilitate clinical translation. To establish benchmark performance for near-infrared fluorescence image-guided surgery, we have developed a single-chip snapshot multispectral imaging system that integrates arrays of bandpass optical filters and six-transistor backside-illuminated pixels to provide RGB-NIR images with low read noise (3.8 e-), high dynamic range (74 dB), and high quantum efficiencies (54% at 800 nm).