Ion-sensitive field-effect transistor (ISFET) sensing array plays a significant role in DNA-based diagnosis and has developed rapidly as the importance of the Point-of-Care devices is increasingly emphasized. However, these devices face several challenges due to its sensing environment and the application requirements, setting limits on the cost, miniaturization and precision of these devices. In this paper, we propose a multi-sensing approach at the pixel level to extract multiple features from the same spatial point, eliminating the need for off-chip sensors. The presented pixel is composed of an ISFET chemical sensor, an image sensor, a moisture sensor and a temperature sensor, all implemented in standard CMOS technology in a compact pixel. The sensing information is acquired simultaneously and modulated on a single waveform thanks to multi-frequency PWM modulation while compensating for both ISFET non-idealities and dark current. To the best of our knowledge, this architecture represents the first multi-sensing pixel capable of extracting 4 features from the same spatial point.