In this paper, the SEU hardened fishbone layouts are proposed and employed to the delay module and slave latch cells, the width of radiation induced transient pulses is analyzed. Without additional consumption of transistors for the basic Double Interlocked Storage Cell (DICE), the obvious enhancements of SEU tolerance for the fishbone layouts are verified under static-mode tests, though the initial clock signal has significant influence on SEU cross sections. The consistent SEU mitigation results of the proposed fishbone cell applied to both the delay module and flip-flop cell indicate that the novel structure may be suitable for harsh radiation environment.