Tracheal malformations such as incomplete/absent tracheal rings induce flaccidity in the tissue causing stenosis in central airway. The short- and long-segmental deficient rings compromise the rigidity of the airway channel leading to collapse. The anomaly can be of varying length, occur in the cervical, middle, or distal region with varying degrees of obstruction. Despite the conservative treatment and interventional procedures, caseloads with restenosis remain high. Quantifying the local tissue mechanical property can directly measure the segment heath and aid in staging the disease. This work discusses the fabrication of MEMS-based piezoresistive force sensor designed for sheep tracheal tissue mechanical characterization.