Details
![Tung-Che Liang Headshot](https://confcats-catavault.s3.amazonaws.com/CATAVault/ieeecass/master/files/styles/cc_user_photo/s3/user-pictures/70771.png?h=2a35d14f&itok=uEbMMKwZ)
- Affiliation
-
AffiliationDuke University
- Country
As microfluidic biochips are being adopted for point-of-care diagnostics, it is critical to ensure bio-sample integrity even before bioassay execution. Molecular barcoding has been proposed recently to thwart sample-forgery attacks. In this work, we present a robust design methodology for molecular barcoding, where the barcodes can be amplified by both PCR and LAMP. We demonstrate the effectiveness of the proposed barcodes using benchtop techniques. While molecular barcoding using traditional PCR can be easily carried out on benchtop settings, we show, for the first time, that molecular barcoding can be validated using LAMP on a fabricated DMFB. The results of LAMP are easy to observe because the workflow includes a color-changing visual indicator. This research opens the door for point-of-care diagnostics using LAMP and microfluidics.